Untargeted Metabolomics Profiling of High Beta Carotene Cassava with respect to Postharvest Physiological Deterioration

Cassava roots undergo postharvest physiological deterioration (PPD), and for most varieties it sets in within 72 hours of harvest. An untargeted metabolomics approach combined with a data-driven statistical analysis was used to characterize profile high beta-carotene cassava the aim identifying any relevant metabolite changes that occur during PPD. Sixteen root samples from four lines were planted greenhouse harvested after months. The included 2 conventionally bred beta carotene – UMUCASS 38, 45 transgenic cultivars - EC20-7 EC20-8 lines. Extracts fresh 20-100 mg tissues analyses data processed using Elements Metabolomics software. Starch lipid metabolites major constituents which may help explain observed differences starch dry matter content among varieties. results provide further insight understanding PPD suggestions on controlling this are made.